BRAF Knockout cell line(HCT116)

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BRAF Knockout cell line(HCT116) Order

BRAF Knockout cell line(HCT116)
Catalog#
YKO-H526
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
BRAF Knockout cell line(HCT116)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
BRAF Knockout cell line (HCT116)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
BRAF
Gene ID
673
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Target region
Knockout region: Exon 3 (based on transcript BRAF-220)
Validation: PCR+Sanger Sequencing
BRAF Knockout cell line(HCT116) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HCT 116
STR Info (Cell bank)
Cell Line:HCT 116
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 10121012
D7S820 11121112
D16S539 11131113
VWA 17221717
TH01 8989
AMEL XXXY
TPOX 8889
CSF1PO 710710
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the BRAF gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, BRAF gene knockout BRAF Knockout cell line (HCT116) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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