CRIP1 Knockout cell line (Hep G2) | Ubigene

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CRIP1 Knockout cell line (Hep G2) Order

CRIP1 Knockout cell line (Hep G2)

Catalog#

YKO-HT7520

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

CRIP1 Knockout cell line (Hep G2)

Detailed Product Information

Product category

KO Cell Lines

cell name

CRIP1 Knockout cell line (Hep G2)

Species

Human

Gene Name

CRIP1

Gene ID

1396

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 3,Exon 4,Exon 5 (based on transcript CRIP1-202)
Validation: PCR+Sanger Sequencing

CRIP1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CRIP1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CRIP1 gene knockout CRIP1 Knockout cell line (Hep G2) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Knockout Cell Line Work Flow and Validatio

CRIP1 Knockout cell line (Hep G2)

CRIP1 Knockout cell line (Hep G2)

Catalog#

YKO-HT7520

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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