CTPS2 Knockout cell line (Hep G2)

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CTPS2 Knockout cell line (Hep G2) Order

CTPS2 Knockout cell line (Hep G2)
Catalog#
YKO-HT7443
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CTPS2 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CTPS2 Knockout cell line (Hep G2)
Species
Human
Gene Name
CTPS2
Gene ID
56474
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript CTPS2-201)
Validation: PCR+Sanger Sequencing
CTPS2 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CTPS2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CTPS2 gene knockout CTPS2 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CTPS2 Knockout cell line (Hep G2)
CTPS2 Knockout cell line (Hep G2)
Catalog#
YKO-HT7443
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • CTPS2 Knockout cell line (HeLa)

    CTPS2 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: CTPS2
    Gene ID: 56474
    Size: 1*10^6
    Catalog#: YKO-XN87253
    YKO-XN87253(HeLa)
  • CTPS2 Knockout cell line (HCT 116)

    CTPS2 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: CTPS2
    Gene ID: 56474
    Size: 1*10^6
    Catalog#: YKO-XN87252
    YKO-XN87252(HCT 116)
  • CTPS2 Knockout cell line (HEK293)

    CTPS2 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: CTPS2
    Gene ID: 56474
    Size: 1*10^6
    Catalog#: YKO-XN87251
    YKO-XN87251(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • CTPS2 Knockout cell line (HeLa)

    CTPS2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: CTPS2
    Gene ID: 56474
    Size: 1*10^6
    Catalog#: YKO-XN87253
      inquiry   More details
  • CTPS2 Knockout cell line (HCT 116)

    CTPS2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: CTPS2
    Gene ID: 56474
    Size: 1*10^6
    Catalog#: YKO-XN87252
      inquiry   More details
  • CTPS2 Knockout cell line (HEK293)

    CTPS2 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: CTPS2
    Gene ID: 56474
    Size: 1*10^6
    Catalog#: YKO-XN87251
      inquiry   More details

CTPS2 Knockout cell line (Hep G2) Order

CTPS2 Knockout cell line (Hep G2)
Catalog#
YKO-HT7443
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CTPS2 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CTPS2 Knockout cell line (Hep G2)
Species
Human
Gene Name
CTPS2
Gene ID
56474
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript CTPS2-201)
Validation: PCR+Sanger Sequencing
CTPS2 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CTPS2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CTPS2 gene knockout CTPS2 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CTPS2 Knockout cell line (Hep G2)
CTPS2 Knockout cell line (Hep G2)
Catalog#
YKO-HT7443
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • CTPS2 Knockout cell line (HeLa)

    CTPS2 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: CTPS2
    Gene ID: 56474
    Size: 1*10^6
    Catalog#: YKO-XN87253
    YKO-XN87253(HeLa)
  • CTPS2 Knockout cell line (HCT 116)

    CTPS2 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: CTPS2
    Gene ID: 56474
    Size: 1*10^6
    Catalog#: YKO-XN87252
    YKO-XN87252(HCT 116)
  • CTPS2 Knockout cell line (HEK293)

    CTPS2 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: CTPS2
    Gene ID: 56474
    Size: 1*10^6
    Catalog#: YKO-XN87251
    YKO-XN87251(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • CTPS2 Knockout cell line (HeLa)

    CTPS2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: CTPS2
    Gene ID: 56474
    Size: 1*10^6
    Catalog#: YKO-XN87253
      inquiry   More details
  • CTPS2 Knockout cell line (HCT 116)

    CTPS2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: CTPS2
    Gene ID: 56474
    Size: 1*10^6
    Catalog#: YKO-XN87252
      inquiry   More details
  • CTPS2 Knockout cell line (HEK293)

    CTPS2 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: CTPS2
    Gene ID: 56474
    Size: 1*10^6
    Catalog#: YKO-XN87251
      inquiry   More details

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