CYP51A1 Knockout cell line (SRA01/04)

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CYP51A1 Knockout cell line (SRA01/04) Order

CYP51A1 Knockout cell line (SRA01/04)
Catalog#
YKO-H799
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CYP51A1 Knockout cell line (SRA01/04)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
CYP51A1 Knockout cell line（SRA01/04）
Cell Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：3
Species
Human
Gene Name
CYP51A1
Gene ID
1595
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
80% DMEM+20%FBS
Accession
CVCL_E1CD(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 1 (based on transcript CYP51A1-201)
Validation: PCR+Sanger Sequencing
CYP51A1 Knockout cell line (SRA01/04) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:SRA01/04		 STR Info (Cell bank)
Cell Line:SRA 01/04
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 111213.0111213.0
D13S317 10101010
D7S820 101113.0101113.0
D16S539 10101010
VWA 17201720
TH01 6767
AMEL XYXY
TPOX 8888
CSF1PO 111315.0111315.0
D12S391 1922
FGA 1922.2
D2S1338 2324
D21S11 2728
D18S51 1313
D8S1179 1415
D3S1358 1415
D6S1043 1116
PENTAE 721
D19S433 1414
PENTAD 91213.0
D1S1656 1316
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CYP51A1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SRA01/04 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CYP51A1 gene knockout CYP51A1 Knockout cell line（SRA01/04） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CYP51A1 Knockout cell line (SRA01/04)
CYP51A1 Knockout cell line (SRA01/04)
Catalog#
YKO-H799
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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