CYP51A1 Knockout cell line (SRA01/04)

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location： Home > Products > KO Cell Lines > CYP51A1 Knockout cell line (SRA01/04)

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CYP51A1 Knockout cell line (SRA01/04) Order

CYP51A1 Knockout cell line (SRA01/04)
Catalog#
YKO-H799
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CYP51A1 Knockout cell line (SRA01/04)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CYP51A1 Knockout cell line (SRA01/04)
Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：3
Species
Human
Gene Name
CYP51A1
Gene ID
1595
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
80% DMEM+20%FBS
Target region
Knockout region: Exon 1 (based on transcript CYP51A1-201)
Validation: PCR+Sanger Sequencing
CYP51A1 Knockout cell line (SRA01/04) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:SRA01/04		 STR Info (Cell bank)
Cell Line:SRA 01/04
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 111213.0111213.0
D13S317 10101010
D7S820 101113.0101113.0
D16S539 10101010
VWA 17201720
TH01 6767
AMEL XYXY
TPOX 8888
CSF1PO 111315.0111315.0
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CYP51A1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SRA01/04 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CYP51A1 gene knockout CYP51A1 Knockout cell line (SRA01/04) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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