DNAJB9 Knockout cell line (Hep G2)

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DNAJB9 Knockout cell line (Hep G2) Order

DNAJB9 Knockout cell line (Hep G2)
Catalog#
YKO-HT7548
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
DNAJB9 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
DNAJB9 Knockout cell line (Hep G2)
Species
Human
Gene Name
DNAJB9
Gene ID
4189
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript DNAJB9-201)
Validation: PCR+Sanger Sequencing
DNAJB9 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the DNAJB9 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, DNAJB9 gene knockout DNAJB9 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
DNAJB9 Knockout cell line (Hep G2)
DNAJB9 Knockout cell line (Hep G2)
Catalog#
YKO-HT7548
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • DNAJB9 Knockout cell line (HeLa)

    DNAJB9 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: DNAJB9
    Gene ID: 4189
    Size: 1*10^6
    Catalog#: YKO-XN30376
    YKO-XN30376(HeLa)
  • DNAJB9 Knockout cell line (HCT 116)

    DNAJB9 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: DNAJB9
    Gene ID: 4189
    Size: 1*10^6
    Catalog#: YKO-XN30375
    YKO-XN30375(HCT 116)
  • DNAJB9 Knockout cell line (HEK293)

    DNAJB9 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: DNAJB9
    Gene ID: 4189
    Size: 1*10^6
    Catalog#: YKO-XN30374
    YKO-XN30374(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • DNAJB9 Knockout cell line (HeLa)

    DNAJB9 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: DNAJB9
    Gene ID: 4189
    Size: 1*10^6
    Catalog#: YKO-XN30376
      inquiry   More details
  • DNAJB9 Knockout cell line (HCT 116)

    DNAJB9 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: DNAJB9
    Gene ID: 4189
    Size: 1*10^6
    Catalog#: YKO-XN30375
      inquiry   More details
  • DNAJB9 Knockout cell line (HEK293)

    DNAJB9 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: DNAJB9
    Gene ID: 4189
    Size: 1*10^6
    Catalog#: YKO-XN30374
      inquiry   More details

DNAJB9 Knockout cell line (Hep G2) Order

DNAJB9 Knockout cell line (Hep G2)
Catalog#
YKO-HT7548
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
DNAJB9 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
DNAJB9 Knockout cell line (Hep G2)
Species
Human
Gene Name
DNAJB9
Gene ID
4189
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript DNAJB9-201)
Validation: PCR+Sanger Sequencing
DNAJB9 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the DNAJB9 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, DNAJB9 gene knockout DNAJB9 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
DNAJB9 Knockout cell line (Hep G2)
DNAJB9 Knockout cell line (Hep G2)
Catalog#
YKO-HT7548
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • DNAJB9 Knockout cell line (HeLa)

    DNAJB9 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: DNAJB9
    Gene ID: 4189
    Size: 1*10^6
    Catalog#: YKO-XN30376
    YKO-XN30376(HeLa)
  • DNAJB9 Knockout cell line (HCT 116)

    DNAJB9 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: DNAJB9
    Gene ID: 4189
    Size: 1*10^6
    Catalog#: YKO-XN30375
    YKO-XN30375(HCT 116)
  • DNAJB9 Knockout cell line (HEK293)

    DNAJB9 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: DNAJB9
    Gene ID: 4189
    Size: 1*10^6
    Catalog#: YKO-XN30374
    YKO-XN30374(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • DNAJB9 Knockout cell line (HeLa)

    DNAJB9 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: DNAJB9
    Gene ID: 4189
    Size: 1*10^6
    Catalog#: YKO-XN30376
      inquiry   More details
  • DNAJB9 Knockout cell line (HCT 116)

    DNAJB9 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: DNAJB9
    Gene ID: 4189
    Size: 1*10^6
    Catalog#: YKO-XN30375
      inquiry   More details
  • DNAJB9 Knockout cell line (HEK293)

    DNAJB9 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: DNAJB9
    Gene ID: 4189
    Size: 1*10^6
    Catalog#: YKO-XN30374
      inquiry   More details

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