EPCAM Knockout cell line (NCI-H292)

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EPCAM Knockout cell line (NCI-H292) Order

EPCAM Knockout cell line (NCI-H292)
Catalog#
YKO-H780
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
EPCAM Knockout cell line (NCI-H292)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
EPCAM Knockout cell line（NCI-H292）
Cell Morphology
Epithelial-like, adherent
Passage ratio
1：2~1：4
Species
Human
Gene Name
EPCAM
Gene ID
4072
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Accession
CVCL_E0ZT(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript EPCAM-201)
Validation: PCR+Sanger Sequencing
EPCAM Knockout cell line (NCI-H292) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:NCI-H292		 STR Info (Cell bank)
Cell Line:NCI-H292 [H292]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11121112
D7S820 10101010
D16S539 913913
VWA 16171617
TH01 8888
AMEL XXXX
TPOX 811811
CSF1PO 10101010
D12S391 1620
FGA 2226
D2S1338 2124
D21S11 2828
D18S51 1616
D8S1179 1114
D3S1358 1616
D6S1043 1112
PENTAE 1315
D19S433 1213.2
PENTAD 99
D1S1656 1415.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the EPCAM gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NCI-H292 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, EPCAM gene knockout EPCAM Knockout cell line（NCI-H292） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
EPCAM Knockout cell line (NCI-H292)
EPCAM Knockout cell line (NCI-H292)
Catalog#
YKO-H780
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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