FABP5 Knockout cell line(MDA-MB-231)

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location： Home > Products > KO Cell Lines > FABP5 Knockout cell line(MDA-MB-231)

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FABP5 Knockout cell line(MDA-MB-231) Order

FABP5 Knockout cell line(MDA-MB-231)
Catalog#
YKO-H509
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
FABP5 Knockout cell line(MDA-MB-231)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
FABP5 Knockout cell line (MDA-MB-231)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
FABP5
Gene ID
2171
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)
Target region
Knockout region: Exon 2 (based on transcript FABP5-201)
Validation: PCR+Sanger Sequencing
FABP5 Knockout cell line(MDA-MB-231) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MDA-MB-231		 STR Info (Cell bank)
Cell Line:MDA-MB-231
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 13131313
D7S820 8989
D16S539 12121212
VWA 15181518
TH01 79.379.3
AMEL XXXX
TPOX 8989
CSF1PO 12131213
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FABP5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MDA-MB-231 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FABP5 gene knockout FABP5 Knockout cell line (MDA-MB-231) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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