FABP5 Knockout cell line(MDA-MB-231)

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Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

FABP5 Knockout cell line(MDA-MB-231) Order

Catalog#：YKO-H509

Price (USD)：-

Size： 1*10^6

Instruction：

Exclusive Red Cotton system（en.rc-crispr.com）was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YKO-H509
Cell line	FABP5 Knockout cell line(MDA-MB-231)	Cell Type	Human Breast Cancer Cell Line
Morphology	Epithelial-like, adherent	Passage ratio	1:3~1:4
Culture method	90%L-15+10%FBS
Cryopreservation solution	90%FBS+10%DMSO
Special Note	L-15 medium is formulated for growth of cells in CO2 free system. If using CO2 incubator, seal the flask cap tightly to ensure a CO2-free environment

STR Validation

Gene Information

Official symbol

FABP5

Gene id

2171

Official full symbol

fatty acid binding protein 5

Also known as

E-FABP, EFABP, KFABP, PA-FABP, PAFABP

This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may play roles in fatty acid uptake, transport, and metabolism. Polymorphisms in this gene are associated with type 2 diabetes. The human genome contains many pseudogenes similar to this locus.