Fat1 Knockout cell line(ID8)

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Fat1 Knockout cell line(ID8) Order

Fat1 Knockout cell line(ID8)
Catalog#
YKO-M151
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Fat1 Knockout cell line(ID8)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Fat1 Knockout cell line (ID8 )
Morphology
Epithelial-like, adherent
Passage ratio
1:3-1:4
Species
Mouse
Gene Name
Fat1
Gene ID
14107
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 1 (based on transcript Fat1-206)
Validation: PCR+Sanger Sequencing
Fat1 Knockout cell line(ID8) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:ID8
STR Info (Cell bank)
Cell Line:ID8
Allele1Allele2Allele3Allele4Allele5Allele1Allele2Allele3
4-2 233.84
【19.3】
19.3
5-5 348.09
【17】
17.0
6-4 300.16
【18】
18.0
6-7 346.66
【15】
15.0
9-2 233.58
【18】
18.0
12-1 230.04
【17】
17.0
15-3 201.09
【22.3】
22.3
18-3 156.24
【17】
17.0
X-1 408.55
【27】
27.0
D4S2408
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Fat1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the ID8 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Fat1 gene knockout Fat1 Knockout cell line (ID8 ) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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