GAL Knockout cell line (HEK293)

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location： Home > Products > KO Cell Lines > GAL Knockout cell line (HEK293)

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GAL Knockout cell line (HEK293) Order

GAL Knockout cell line (HEK293)
Catalog#
YKO-HS10653
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GAL Knockout cell line (HEK293)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GAL Knockout cell line (HEK293)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
GAL
Gene ID
51083
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 3 (based on transcript GAL-201)
Validation: PCR+Sanger Sequencing
GAL Knockout cell line (HEK293) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GAL gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GAL gene knockout GAL Knockout cell line (HEK293) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GAL Knockout cell line (HEK293)
GAL Knockout cell line (HEK293)
Catalog#
YKO-HS10653
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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