GPNMB Knockout cell line (THP-1)

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Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

GPNMB Knockout cell line (THP-1) Order

Catalog#：YKO-H623

Price (USD)：-

Size： 1*10^6

Instruction：

Exclusive Red Cotton system（en.rc-crispr.com）was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YKO-H623
Cell line	GPNMB Knockout cell line(THP-1)	Cell Type	Human Ovarian Granulosa Cell Line
Morphology	Monocyte, suspension	Passage ratio	Control the cell density at 2x10^5~4.0x10^5 cells/ml during passaging, and carry out the passage when the cells grow to 8x10^5~1.0x10^6 cells/ml.
Culture method	90%RPMI-1640+10%FBS
Cryopreservation solution	90%FBS+10%DMSO
Special Note	The cell culture density needs to be strictly controlled. The cell viability after thawing is relatively low, it is recommended to use Australia-sourced FBS for thawing or increase the FBS ratio to 20%. When the cells grow normally and can be passaged after 2 passages, the cells can be cultured using the normal culture method.

STR Validation

Gene Information

Official symbol

GPNMB

Gene id

10457

Official full symbol

glycoprotein nmb

Also known as

HGFIN, NMB, PLCA3

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.