GPR75 Knockout cell line (HEK293)

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GPR75 Knockout cell line (HEK293) Order

GPR75 Knockout cell line (HEK293)
Catalog#
YKO-H708
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GPR75 Knockout cell line (HEK293)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GPR75 Knockout cell line (HEK293)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
GPR75
Gene ID
10936
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 2 (based on transcript GPR75-201)
Validation: PCR+Sanger Sequencing
GPR75 Knockout cell line (HEK293) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GPR75 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GPR75 gene knockout GPR75 Knockout cell line (HEK293) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GPR75 Knockout cell line (HEK293)
GPR75 Knockout cell line (HEK293)
Catalog#
YKO-H708
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • GPR75 Knockout cell line (HeLa)

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    Organism: Human
    Gene Name: GPR75
    Gene ID: 10936
    Size: 1*10^6
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  • GPR75 Knockout cell line (HCT 116)

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    Gene Name: GPR75
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