GRK2 Knockout cell line(HEK293)

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location: Home > Products > KO Cell Lines > GRK2 Knockout cell line(HEK293)
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GRK2 Knockout cell line(HEK293) Order

GRK2 Knockout cell line(HEK293)
Catalog#
YKO-H597
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GRK2 Knockout cell line(HEK293)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GRK2 Knockout cell line (HEK293)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
GRK2
Gene ID
156
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 2 (based on transcript GRK2-201)
Validation: PCR+Sanger Sequencing
GRK2 Knockout cell line(HEK293) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GRK2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GRK2 gene knockout GRK2 Knockout cell line (HEK293) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GRK2 Knockout cell line(HEK293)
GRK2 Knockout cell line(HEK293)
Catalog#
YKO-H597
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Organism: Human
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    Gene ID: 156
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    Gene Name: GRK2
    Gene ID: 156
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