Gpx4 Knockout cell line (MDCK(NBL-2))

EN CN
Scientist.com
Approved Supplier
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
ENCN
location： Home > Products > KO Cell Lines > Gpx4 Knockout cell line (MDCK(NBL-2))
KO Cell Lines
Stable Cell Lines
Wild-type Cell Lines
Stem Cell Lines
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • OVA Stable Cell Lines
  • Stable Cell Lines
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

Gpx4 Knockout cell line (MDCK(NBL-2)) Order

Gpx4 Knockout cell line (MDCK(NBL-2))
Catalog#
YKO-M156
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Gpx4 Knockout cell line (MDCK(NBL-2))
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Gpx4 Knockout cell line (MDCK(NBL-2))
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Mouse
Gene Name
Gpx4
Gene ID
625249
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
89%MEM+10%FBS+1% NEAA
Accession
CVCL_E0Z8(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 1 (based on transcript Gpx4-201)
Validation: PCR+Sanger Sequencing
Gpx4 Knockout cell line (MDCK(NBL-2)) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Gpx4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MDCK(NBL-2) cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Gpx4 gene knockout Gpx4 Knockout cell line (MDCK(NBL-2)) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Gpx4 Knockout cell line (MDCK(NBL-2))
Gpx4 Knockout cell line (MDCK(NBL-2))
Catalog#
YKO-M156
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • GPX4 Knockout cell line (HCT 116)

    GPX4 Knockout cell line (HCT 116)
    Request a Quote More details

    Organism:
    Gene Name: GPX4
    Gene ID: 2879
    Size: 1*10^6
    Catalog#: YKO-H1476
    YKO-H1476(HCT 116)
  • GPX4 Knockout cell line (HCT 116)

    GPX4 Knockout cell line (HCT 116)
    Request a Quote More details

    Organism: Human
    Gene Name: GPX4
    Gene ID: 2879
    Size: 1*10^6
    Catalog#: YKO-KP248
    YKO-KP248(HCT 116)
  • Gpx4 Knockout cell line (RAW 264.7)

    Gpx4 Knockout cell line (RAW 264.7)
    Request a Quote More details

    Organism: Mouse
    Gene Name: Gpx4
    Gene ID: 625249
    Size: 1*10^6
    Catalog#: YKO-M109
    YKO-M109(RAW 264.7)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines


WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now
Contact us
✖

Contact us

If we are unable to reach you via email, how else can we contact you?
✖
Submission Succeeded
Book A Virtual Meeting With Technical Support