HIF1A Knockout cell line (L-02)

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HIF1A Knockout cell line (L-02) (Hela-contaminated cells, not for sales) Order

HIF1A Knockout cell line  (L-02)
Catalog#
YKO-H877
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
HIF1A Knockout cell line  (L-02)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
HIF1A Knockout cell line (L-02)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
HIF1A
Gene ID
3091
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
80% RPMI-1640+20% FBS
Target region
Knockout region: Exon 2 (based on transcript HIF1A-202)
Validation: PCR+Sanger Sequencing
HIF1A Knockout cell line  (L-02) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:L-02
STR Info (Cell bank)
Cell Line:L-02
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 13.313.313.313.3
D7S820 12121212
D16S539 910910
VWA 16161618
TH01 7777
AMEL XXXX
TPOX 12121212
CSF1PO 10101010
* Hela-contaminated cells, not for sales
Reviewed by ICLAC (ref: 190616). L-02 was reported to be established from normal liver cells but its STR profile corresponds to HeLa. Samples tested in this study were directly deposited in the cell line repository that performed the investigation. L-02 has a derivative: HL-7702BaPT. No STR profiling information was available for the derivative at the time of review.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the HIF1A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the L-02 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, HIF1A gene knockout HIF1A Knockout cell line (L-02) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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