IGF2BP2 Knockout cell line(786-O)

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IGF2BP2 Knockout cell line(786-O) Order

IGF2BP2 Knockout cell line(786-O)
Catalog#
YKO-H496
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
IGF2BP2 Knockout cell line(786-O)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
IGF2BP2 Knockout cell line (786-O)
Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:4
Species
Human
Gene Name
IGF2BP2
Gene ID
10644
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 5 (based on transcript IGF2BP2-202)
Validation: PCR+Sanger Sequencing
IGF2BP2 Knockout cell line(786-O) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:786-O
STR Info (Cell bank)
Cell Line:786-O [786-0]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 9999
D13S317 8888
D7S820 11121112
D16S539 12121212
VWA 15171517
TH01 69.369.3
AMEL XYXY
TPOX 811811
CSF1PO 10101010
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the IGF2BP2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 786-O cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, IGF2BP2 gene knockout IGF2BP2 Knockout cell line (786-O) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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