JTB Knockout cell line (Hep G2)

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JTB Knockout cell line (Hep G2) Order

JTB Knockout cell line (Hep G2)
Catalog#
YKO-HT7560
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
JTB Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
JTB Knockout cell line (Hep G2)
Species
Human
Gene Name
JTB
Gene ID
10899
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript JTB-201)
Validation: PCR+Sanger Sequencing
JTB Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the JTB gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, JTB gene knockout JTB Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
JTB Knockout cell line (Hep G2)
JTB Knockout cell line (Hep G2)
Catalog#
YKO-HT7560
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • JTB Knockout cell line (HeLa)

    JTB Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: JTB
    Gene ID: 10899
    Size: 1*10^6
    Catalog#: YKO-XN45032
    YKO-XN45032(HeLa)
  • JTB Knockout cell line (HCT 116)

    JTB Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: JTB
    Gene ID: 10899
    Size: 1*10^6
    Catalog#: YKO-XN45031
    YKO-XN45031(HCT 116)
  • JTB Knockout cell line (HEK293)

    JTB Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: JTB
    Gene ID: 10899
    Size: 1*10^6
    Catalog#: YKO-XN45030
    YKO-XN45030(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • JTB Knockout cell line (HeLa)

    JTB Knockout cell line (HeLa)

    Organism: Human
    Gene Name: JTB
    Gene ID: 10899
    Size: 1*10^6
    Catalog#: YKO-XN45032
      inquiry   More details
  • JTB Knockout cell line (HCT 116)

    JTB Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: JTB
    Gene ID: 10899
    Size: 1*10^6
    Catalog#: YKO-XN45031
      inquiry   More details
  • JTB Knockout cell line (HEK293)

    JTB Knockout cell line (HEK293)

    Organism: Human
    Gene Name: JTB
    Gene ID: 10899
    Size: 1*10^6
    Catalog#: YKO-XN45030
      inquiry   More details

JTB Knockout cell line (Hep G2) Order

JTB Knockout cell line (Hep G2)
Catalog#
YKO-HT7560
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
JTB Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
JTB Knockout cell line (Hep G2)
Species
Human
Gene Name
JTB
Gene ID
10899
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript JTB-201)
Validation: PCR+Sanger Sequencing
JTB Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the JTB gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, JTB gene knockout JTB Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
JTB Knockout cell line (Hep G2)
JTB Knockout cell line (Hep G2)
Catalog#
YKO-HT7560
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • JTB Knockout cell line (HeLa)

    JTB Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: JTB
    Gene ID: 10899
    Size: 1*10^6
    Catalog#: YKO-XN45032
    YKO-XN45032(HeLa)
  • JTB Knockout cell line (HCT 116)

    JTB Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: JTB
    Gene ID: 10899
    Size: 1*10^6
    Catalog#: YKO-XN45031
    YKO-XN45031(HCT 116)
  • JTB Knockout cell line (HEK293)

    JTB Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: JTB
    Gene ID: 10899
    Size: 1*10^6
    Catalog#: YKO-XN45030
    YKO-XN45030(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • JTB Knockout cell line (HeLa)

    JTB Knockout cell line (HeLa)

    Organism: Human
    Gene Name: JTB
    Gene ID: 10899
    Size: 1*10^6
    Catalog#: YKO-XN45032
      inquiry   More details
  • JTB Knockout cell line (HCT 116)

    JTB Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: JTB
    Gene ID: 10899
    Size: 1*10^6
    Catalog#: YKO-XN45031
      inquiry   More details
  • JTB Knockout cell line (HEK293)

    JTB Knockout cell line (HEK293)

    Organism: Human
    Gene Name: JTB
    Gene ID: 10899
    Size: 1*10^6
    Catalog#: YKO-XN45030
      inquiry   More details

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