KMT2D Knockout cell line (MS751)

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Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

KMT2D Knockout cell line (MS751) Order

Catalog#：YKO-H766

Price (USD)：-

Size： 1*10^6

Instruction：

Exclusive Red Cotton system（en.rc-crispr.com）was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YKO-H766
Cell line	KMT2D Knockout cell line(MS751)	Cell Type	Human Cervical Epidermal Cell Line
Morphology	Epithelial-like, adherent	Passage ratio	1:2-1:3
Culture method	88%MEM+10%FBS+1%NEAA+1%Sodium Pyruvate
Cryopreservation solution	90%FBS+10%DMSO
Special Note

STR Validation

Gene Information

Official symbol

KMT2D

Gene id

8085

Official full symbol

lysine methyltransferase 2D

Also known as

AAD10, ALR, CAGL114, KABUK1, KMS, MLL2, MLL4, TNRC21

The protein encoded by this gene is a histone methyltransferase that methylates the Lys-4 position of histone H3. The encoded protein is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the beta-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome.