KRTAP4-16 Knockout cell line (Hep G2)

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KRTAP4-16 Knockout cell line (Hep G2) Order

KRTAP4-16 Knockout cell line (Hep G2)
Catalog#
YKO-HT7660
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
KRTAP4-16 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
KRTAP4-16 Knockout cell line (Hep G2)
Species
Human
Gene Name
KRTAP4-16
Gene ID
85354
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the KRTAP4-16 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, KRTAP4-16 gene knockout KRTAP4-16 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
KRTAP4-16 Knockout cell line (Hep G2)
KRTAP4-16 Knockout cell line (Hep G2)
Catalog#
YKO-HT7660
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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