LZTR1 Knockout cell line (HEP3B2.1-7)

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Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

LZTR1 Knockout cell line (HEP3B2.1-7) Order

Catalog#：YKO-H844

Price (USD)：-

Size： 1*10^6

Instruction：

Exclusive Red Cotton system（en.rc-crispr.com）was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YKO-H844
Cell line	LZTR1 Knockout cell line (HEP3B2.1-7)	Cell Type	Human Hepatoma Cell Line
Morphology	Epithelial-like, adherent	Passage ratio	1:3~1:5
Culture method	87%MEM+10% FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA
Cryopreservation solution	90%FBS+10%DMSO
Special Note

Gene Information

Official symbol

LZTR1

Gene id

8216

Official full symbol

leucine zipper like transcription regulator 1

Also known as

BTBD29, LZTR-1, NS10, NS2, SWNTS2

This gene encodes a member of the BTB-kelch superfamily. Initially described as a putative transcriptional regulator based on weak homology to members of the basic leucine zipper-like family, the encoded protein subsequently has been shown to localize exclusively to the Golgi network where it may help stabilize the Gogli complex. Deletion of this gene may be associated with DiGeorge syndrome.