MAVS Knockout cell line (293T)

location: Home > Products > KO Cell Lines > MAVS Knockout cell line (293T)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus
MAVS Knockout cell line (293T)

MAVS Knockout cell line (293T) Order

Catalog#YKO-H840

Price (USD)-

Size 1*10^6

Instruction

Exclusive Red Cotton system(en.rc-crispr.com)was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Cell Info

Catalog YKO-H840
Cell line MAVS Knockout cell line (293T) Cell Type Human Embryonic Kidney Cell Line
Morphology Epithelial-like, adherent Passage ratio 1:3~1:6
Culture method 90% DMEM+10%FBS
Cryopreservation solution 50%DMEM+40%FBS+10%DMSO
Special Note

STR Validation

Gene Information

Official symbol
MAVS
Gene id
57506
Official full symbol
mitochondrial antiviral signaling protein
Also known as
CARDIF, IPS-1, IPS1, VISA
This gene encodes an intermediary protein necessary in the virus-triggered beta interferon signaling pathways. It is required for activation of transcription factors which regulate expression of beta interferon and contributes to antiviral innate immunity.

KO strategy

Knockout region: Exon 6 (based on transcript MAVS-202)
Validation: PCR+Sanger Sequencing

Method

Exclusive Red Cotton system(en.rc-crispr.com)was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Contact us