MCOLN1 Knockout cell line (T98G)

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Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

MCOLN1 Knockout cell line (T98G) Order

Catalog#：YKO-H792

Price (USD)：-

Size： 1*10^6

Instruction：

Exclusive Red Cotton system（en.rc-crispr.com）was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YKO-H792
Cell line	MCOLN1 Knockout cell line (T98G)	Cell Type	T98G Cell Line
Morphology	Fibroblast，adherent	Passage ratio	1:2-1:4
Culture method	88%MEM+10%FBS+1%NEAA+1%Sodium Pyruvate 100 mM Solution
Cryopreservation solution	90%FBS+10%DMSO
Special Note

Gene Information

Official symbol

MCOLN1

Gene id

57192

Official full symbol

mucolipin TRP cation channel 1

Also known as

MG-2, ML1, ML4, MLIV, MST080, MSTP080, TRP-ML1, TRPM-L1, TRPML1

This gene encodes a memberof the transient receptor potential (TRP) cation channel gene family. The transmembrane protein localizes to intracellular vesicular membranes including lysosomes, and functions in the late endocytic pathway and in the regulation of lysosomal exocytosis. The channel is permeable to Ca(2+), Fe(2+), Na(+), K(+), and H(+), and is modulated by changes in Ca(2+) concentration. Mutations in this gene result in mucolipidosis type IV.