MTRR Knockout cell line (HepG2)

location： Home > Products > KO Cell Lines > MTRR Knockout cell line (HepG2)

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

MTRR Knockout cell line (HepG2) Order

Catalog#：YKO-H826

Price (USD)：-

Size： 1*10^6

Instruction：

Exclusive Red Cotton system（en.rc-crispr.com）was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YKO-H826
Cell line	MTRR Knockout cell line (HepG2)	Cell Type	Human Hepatoma Cell Line
Morphology	Epithelial-like, adherent	Passage ratio	1:3~1:5
Culture method	90% DMEM+10% FBS
Cryopreservation solution	50%DMEM+40%FBS+10%DMSO
Special Note

STR Validation

Gene Information

Official symbol

MTRR

Gene id

4552

Official full symbol

5-methyltetrahydrofolate-homocysteine methyltransferase reductase

Also known as

MSR, cblE

This gene encodes a member of the ferredoxin-NADP(+) reductase (FNR) family of electron transferases. This protein functions in the synthesis of methionine by regenerating methionine synthase to a functional state. Because methionine synthesis requires methyl-group transfer by a folate donor, activity of the encoded enzyme is important for folate metabolism and cellular methylation. Mutations in this gene can cause homocystinuria-megaloblastic anemia, cbl E type. Alternative splicing of this gene results in multiple transcript variants.