CXCR4 Knockout cell line(U-2 OS)

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CXCR4 Knockout cell line(U-2 OS) Order

CXCR4 Knockout cell line(U-2 OS)
Catalog#
YKO-H443
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CXCR4 Knockout cell line(U-2 OS)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CXCR4 Knockout cell line (U-2 OS)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:5
Species
Human
Gene Name
CXCR4
Gene ID
7852
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% McCOY's 5A+10%FBS
Target region
Knockout region: Exon 2 (based on transcript CXCR4-201)
Validation: PCR+Sanger Sequencing
CXCR4 Knockout cell line(U-2 OS) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:U-2 OS
STR Info (Cell bank)
Cell Line:U-2 OS
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 13131313
D7S820 11121112
D16S539 11121112
VWA 14181418
TH01 69.369.3
AMEL XXXX
TPOX 11121112
CSF1PO 13131313
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CXCR4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-2 OS cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CXCR4 gene knockout CXCR4 Knockout cell line (U-2 OS) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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