NCAPD3 Knockout cell line (Hep G2)

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NCAPD3 Knockout cell line (Hep G2) Order

NCAPD3 Knockout cell line (Hep G2)
Catalog#
YKO-HT7599
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NCAPD3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
NCAPD3 Knockout cell line (Hep G2)
Species
Human
Gene Name
NCAPD3
Gene ID
23310
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript NCAPD3-212)
Validation: PCR+Sanger Sequencing
NCAPD3 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NCAPD3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NCAPD3 gene knockout NCAPD3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
NCAPD3 Knockout cell line (Hep G2)
NCAPD3 Knockout cell line (Hep G2)
Catalog#
YKO-HT7599
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

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KO Cell Lines

  • NCAPD3 Knockout cell line (Jurkat)

    NCAPD3 Knockout cell line (Jurkat)

    Organism: Human
    Gene Name: NCAPD3
    Gene ID: 23310
    Size: 1*10^6
    Catalog#: YKO-HT9652
    Request a Quote More details
  • NCAPD3 Knockout cell line (HeLa)

    NCAPD3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: NCAPD3
    Gene ID: 23310
    Size: 1*10^6
    Catalog#: YKO-HS14680
    Request a Quote More details
  • NCAPD3 Knockout cell line (HCT 116)

    NCAPD3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: NCAPD3
    Gene ID: 23310
    Size: 1*10^6
    Catalog#: YKO-HS14679
    Request a Quote More details

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