NF1 Knockout cell line (U-87 MG)

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NF1 Knockout cell line (U-87 MG) Order

NF1 Knockout cell line (U-87 MG)
Catalog#
YKO-H833
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NF1 Knockout cell line (U-87 MG)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NF1 Knockout cell line (U-87 MG)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
NF1
Gene ID
4763
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 12 (based on transcript NF1-202)
Validation: PCR+Sanger Sequencing
NF1 Knockout cell line (U-87 MG) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:U-87 MG		 STR Info (Cell bank)
Cell Line:U-87MG
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 811811
D7S820 8989
D16S539 12121212
VWA 15171517
TH01 9.39.39.39.3
AMEL XXXX
TPOX 8888
CSF1PO 10111011
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NF1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-87 MG cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NF1 gene knockout NF1 Knockout cell line (U-87 MG) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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