PACRGL Knockout cell line (Hep G2)

Gene Editing Services

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Red Cotton Gene knockout Project

PACRGL Knockout cell line (Hep G2) Order

Catalog#

YKO-HT7492

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

PACRGL Knockout cell line (Hep G2)

Species

Human

Gene Name

PACRGL

Gene ID

133015

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 3 (based on transcript PACRGL-208)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PACRGL gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PACRGL gene knockout PACRGL Knockout cell line (Hep G2) will be expanded and cryopreserved.