PCSK5 Knockout cell line (Jurkat)

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PCSK5 Knockout cell line (Jurkat) Order

PCSK5 Knockout cell line (Jurkat)
Catalog#
YKO-H1444
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PCSK5 Knockout cell line (Jurkat)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
PCSK5 Knockout cell line (Jurkat)
Species
Gene Name
PCSK5
Gene ID
5125
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript PCSK5-207)
Validation: PCR+Sanger Sequencing
PCSK5 Knockout cell line (Jurkat) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PCSK5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Jurkat cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PCSK5 gene knockout PCSK5 Knockout cell line (Jurkat) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PCSK5 Knockout cell line (Jurkat)
PCSK5 Knockout cell line (Jurkat)
Catalog#
YKO-H1444
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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