POLD1 Knockout cell line (A549)

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Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

POLD1 Knockout cell line (A549) Order

Catalog#：YKO-H791

Price (USD)：-

Size： 1*10^6

Instruction：

Exclusive Red Cotton system（en.rc-crispr.com）was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YKO-H791
Cell line	POLD1 Knockout cell line (A549)	Cell Type	Human Lung Cancer Cell Line
Morphology	Epithelial-like, adherent	Passage ratio	1:3~1:5
Culture method	90% F12K+10% FBS
Cryopreservation solution	50%F12K+40%FBS+10%DMSO
Special Note

STR Validation

Gene Information

Official symbol

POLD1

Gene id

5424

Official full symbol

DNA polymerase delta 1, catalytic subunit

Also known as

CDC2, CRCS10, MDPL, POLD

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.