PPP1R12B Knockout cell line(Hep G2)

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PPP1R12B Knockout cell line(Hep G2) Order

PPP1R12B Knockout cell line(Hep G2)
Catalog#
YKO-H427
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PPP1R12B Knockout cell line(Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PPP1R12B Knockout cell line (Hep G2)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
PPP1R12B
Gene ID
4660
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10% FBS
Target region
Knockout region: Exon 1 (based on transcript PPP1R12B-216)
Validation: PCR+Sanger Sequencing
PPP1R12B Knockout cell line(Hep G2) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:Hep G2
STR Info (Cell bank)
Cell Line:GS-HepG2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 913913
D7S820 10101010
D16S539 12121212
VWA 17171717
TH01 9999
AMEL XYXY
TPOX 8989
CSF1PO 10111011
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PPP1R12B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PPP1R12B gene knockout PPP1R12B Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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