RABL6 Knockout cell line (MCF7)

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RABL6 Knockout cell line (MCF7) Order

RABL6 Knockout cell line (MCF7)
Catalog#
YKO-H1117
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RABL6 Knockout cell line (MCF7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RABL6 Knockout cell line (MCF7)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
RABL6
Gene ID
55684
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
87%MEM+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA
Target region
Knockout region: Exon 8,Exon 9,Exon 10,Exon 11 (based on transcript RABL6-201)
Validation: PCR+Sanger Sequencing
RABL6 Knockout cell line (MCF7) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:MCF7
STR Info (Cell bank)
Cell Line:MCF-7
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11111111
D7S820 8989
D16S539 11121112
VWA 14151415
TH01 6666
AMEL XXXX
TPOX 912912
CSF1PO 10101010
D12S391 1820
FGA 2325
D2S1338 2123
D21S11 3030
D18S51 1414
D8S1179 1014
D3S1358 1616
D6S1043 1218
PENTAE 712
D19S433 1313
PENTAD 1212
D1S1656 1115.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RABL6 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MCF7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RABL6 gene knockout RABL6 Knockout cell line (MCF7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RABL6 Knockout cell line (MCF7)
RABL6 Knockout cell line (MCF7)
Catalog#
YKO-H1117
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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