RNF114 Knockout cell line (MDA-MB-231)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > RNF114 Knockout cell line (MDA-MB-231)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

RNF114 Knockout cell line (MDA-MB-231) Order

RNF114 Knockout cell line (MDA-MB-231)
Catalog#
YKO-H699
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RNF114 Knockout cell line (MDA-MB-231)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RNF114 Knockout cell line(MDA-MB-231)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
RNF114
Gene ID
55905
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)
Target region
Knockout region: Exon 2 (based on transcript RNF114-201)
Validation: PCR+Sanger Sequencing
RNF114 Knockout cell line (MDA-MB-231) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:MDA-MB-231
STR Info (Cell bank)
Cell Line:MDA-MB-231
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 13131313
D7S820 8989
D16S539 12121212
VWA 15181518
TH01 79.379.3
AMEL XXXX
TPOX 8989
CSF1PO 12131213
D12S391 1718
FGA 2223
D2S1338 2021
D21S11 33.233.2
D18S51 1116
D8S1179 1313
D3S1358 1616
D6S1043 1818
PENTAE 1111
D19S433 1114
PENTAD 1114
D1S1656 1517
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RNF114 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MDA-MB-231 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RNF114 gene knockout RNF114 Knockout cell line(MDA-MB-231) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RNF114 Knockout cell line (MDA-MB-231)
RNF114 Knockout cell line (MDA-MB-231)
Catalog#
YKO-H699
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • RNF114 Knockout cell line (HeLa)

    RNF114 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: RNF114
    Gene ID: 55905
    Size: 1*10^6
    Catalog#: YKO-HS10918
    YKO-HS10918(HeLa)
  • RNF114 Knockout cell line (HCT 116)

    RNF114 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: RNF114
    Gene ID: 55905
    Size: 1*10^6
    Catalog#: YKO-HS10917
    YKO-HS10917(HCT 116)
  • RNF114 Knockout cell line (HEK293)

    RNF114 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: RNF114
    Gene ID: 55905
    Size: 1*10^6
    Catalog#: YKO-HS10916
    YKO-HS10916(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • RNF114 Knockout cell line (HeLa)

    RNF114 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: RNF114
    Gene ID: 55905
    Size: 1*10^6
    Catalog#: YKO-HS10918
  • RNF114 Knockout cell line (HCT 116)

    RNF114 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: RNF114
    Gene ID: 55905
    Size: 1*10^6
    Catalog#: YKO-HS10917
  • RNF114 Knockout cell line (HEK293)

    RNF114 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: RNF114
    Gene ID: 55905
    Size: 1*10^6
    Catalog#: YKO-HS10916

Contact us