RNF213 Knockout cell line (HCMEC/D3)

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RNF213 Knockout cell line (HCMEC/D3) Order

RNF213 Knockout cell line (HCMEC/D3)
Catalog#
YKO-H622
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RNF213 Knockout cell line (HCMEC/D3)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RNF213 Knockout cell line (HCMEC/D3)
Morphology
Epithelial-like,?adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
RNF213
Gene ID
57674
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
Special media
Target region
Knockout region: Exon 4 (based on transcript RNF213-220)
Validation: PCR+Sanger Sequencing
RNF213 Knockout cell line (HCMEC/D3) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HCMEC/D3
STR Info (Cell bank)
Cell Line:hCMEC/D3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11141114
D7S820 10121012
D16S539 912912
VWA 14171417
TH01 7979
AMEL XXXX
TPOX 10111011
CSF1PO 10121012
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RNF213 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCMEC/D3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RNF213 gene knockout RNF213 Knockout cell line (HCMEC/D3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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