RTL8C Knockout cell line (Hep G2)

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RTL8C Knockout cell line (Hep G2) Order

RTL8C Knockout cell line (Hep G2)
Catalog#
YKO-HT7654
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RTL8C Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RTL8C Knockout cell line (Hep G2)
Species
Human
Gene Name
RTL8C
Gene ID
8933
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript RTL8C-201)
Validation: PCR+Sanger Sequencing
RTL8C Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RTL8C gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RTL8C gene knockout RTL8C Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RTL8C Knockout cell line (Hep G2)
RTL8C Knockout cell line (Hep G2)
Catalog#
YKO-HT7654
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • RTL8C Knockout cell line (HeLa)

    RTL8C Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: RTL8C
    Gene ID: 8933
    Size: 1*10^6
    Catalog#: YKO-XN40596
    YKO-XN40596(HeLa)
  • RTL8C Knockout cell line (HCT 116)

    RTL8C Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: RTL8C
    Gene ID: 8933
    Size: 1*10^6
    Catalog#: YKO-XN40595
    YKO-XN40595(HCT 116)
  • RTL8C Knockout cell line (HEK293)

    RTL8C Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: RTL8C
    Gene ID: 8933
    Size: 1*10^6
    Catalog#: YKO-XN40594
    YKO-XN40594(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • RTL8C Knockout cell line (HeLa)

    RTL8C Knockout cell line (HeLa)

    Organism: Human
    Gene Name: RTL8C
    Gene ID: 8933
    Size: 1*10^6
    Catalog#: YKO-XN40596
      inquiry   More details
  • RTL8C Knockout cell line (HCT 116)

    RTL8C Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: RTL8C
    Gene ID: 8933
    Size: 1*10^6
    Catalog#: YKO-XN40595
      inquiry   More details
  • RTL8C Knockout cell line (HEK293)

    RTL8C Knockout cell line (HEK293)

    Organism: Human
    Gene Name: RTL8C
    Gene ID: 8933
    Size: 1*10^6
    Catalog#: YKO-XN40594
      inquiry   More details

RTL8C Knockout cell line (Hep G2) Order

RTL8C Knockout cell line (Hep G2)
Catalog#
YKO-HT7654
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RTL8C Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RTL8C Knockout cell line (Hep G2)
Species
Human
Gene Name
RTL8C
Gene ID
8933
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript RTL8C-201)
Validation: PCR+Sanger Sequencing
RTL8C Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RTL8C gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RTL8C gene knockout RTL8C Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RTL8C Knockout cell line (Hep G2)
RTL8C Knockout cell line (Hep G2)
Catalog#
YKO-HT7654
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • RTL8C Knockout cell line (HeLa)

    RTL8C Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: RTL8C
    Gene ID: 8933
    Size: 1*10^6
    Catalog#: YKO-XN40596
    YKO-XN40596(HeLa)
  • RTL8C Knockout cell line (HCT 116)

    RTL8C Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: RTL8C
    Gene ID: 8933
    Size: 1*10^6
    Catalog#: YKO-XN40595
    YKO-XN40595(HCT 116)
  • RTL8C Knockout cell line (HEK293)

    RTL8C Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: RTL8C
    Gene ID: 8933
    Size: 1*10^6
    Catalog#: YKO-XN40594
    YKO-XN40594(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • RTL8C Knockout cell line (HeLa)

    RTL8C Knockout cell line (HeLa)

    Organism: Human
    Gene Name: RTL8C
    Gene ID: 8933
    Size: 1*10^6
    Catalog#: YKO-XN40596
      inquiry   More details
  • RTL8C Knockout cell line (HCT 116)

    RTL8C Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: RTL8C
    Gene ID: 8933
    Size: 1*10^6
    Catalog#: YKO-XN40595
      inquiry   More details
  • RTL8C Knockout cell line (HEK293)

    RTL8C Knockout cell line (HEK293)

    Organism: Human
    Gene Name: RTL8C
    Gene ID: 8933
    Size: 1*10^6
    Catalog#: YKO-XN40594
      inquiry   More details

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