RXRA Knockout cell line(5637)

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RXRA Knockout cell line(5637) Order

RXRA Knockout cell line(5637)
Catalog#
YKO-H536
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RXRA Knockout cell line(5637)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RXRA Knockout cell line (5637)
Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Gene Name
RXRA
Gene ID
6256
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 2 (based on transcript RXRA-202)
Validation: PCR+Sanger Sequencing
RXRA Knockout cell line(5637) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:5637
STR Info (Cell bank)
Cell Line:5637
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11111111
D7S820 10111011
D16S539 9999
VWA 18181818
TH01 7979
AMEL XXXY
TPOX 8889
CSF1PO 11111111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RXRA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 5637 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RXRA gene knockout RXRA Knockout cell line (5637) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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