SIGLEC7 Knockout cell line(H1)

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SIGLEC7 Knockout cell line(H1) Order

SIGLEC7 Knockout cell line(H1)
Catalog#
YKO-H359
Size
2*10^5
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SIGLEC7 Knockout cell line(H1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SIGLEC7 Knockout cell line (H1)
Morphology
Spherical clone
Passage ratio
1:6-1:8
Species
Human
Gene Name
SIGLEC7
Gene ID
27036
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
Target region
Knockout region: Exon 1 (based on transcript SIGLEC7-202)
Validation: PCR+Sanger Sequencing
SIGLEC7 Knockout cell line(H1) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:H1
STR Info (Cell bank)
Cell Line:H1.NPC-i3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 911911
D13S317 811811
D7S820 812812
D16S539 913913
VWA 15171517
TH01 9.39.39.39.3
AMEL XYXY
TPOX 811811
CSF1PO 12131213
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SIGLEC7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SIGLEC7 gene knockout SIGLEC7 Knockout cell line (H1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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