STK11 Knockout cell line (HCT116)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > STK11 Knockout cell line (HCT116)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

STK11 Knockout cell line (HCT116) Order

STK11 Knockout cell line (HCT116)
Catalog#
YKO-H956
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
STK11 Knockout cell line (HCT116)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
STK11 Knockout cell line (HCT116)
Species
Human
Gene Name
STK11
Gene ID
6794
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript STK11-201)
Validation: PCR+Sanger Sequencing
STK11 Knockout cell line (HCT116) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the STK11 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, STK11 gene knockout STK11 Knockout cell line (HCT116) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

KO Cell Lines

  • STK11 Knockout cell line (Hela)

    STK11 Knockout cell line (Hela)

    Organism: Human
    Gene Name: STK11
    Gene ID: 6794
    Size: 1*10^6
    Catalog#: YKO-HS4080
    YKO-HS4080(Hela)
  • STK11 Knockout cell line (HEK293)

    STK11 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: STK11
    Gene ID: 6794
    Size: 1*10^6
    Catalog#: YKO-HS1466
    YKO-HS1466(HEK293)
  • STK11 Knockout cell line (A549)

    STK11 Knockout cell line (A549)

    Organism: Human
    Gene Name: STK11
    Gene ID: 6794
    Size: 1*10^6
    Catalog#: YKO-HS709
    YKO-HS709(A549)

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • STK11 Knockout cell line (Hela)

    STK11 Knockout cell line (Hela)

    Organism: Human
    Gene Name: STK11
    Gene ID: 6794
    Size: 1*10^6
    Catalog#: YKO-HS4080
  • STK11 Knockout cell line (HEK293)

    STK11 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: STK11
    Gene ID: 6794
    Size: 1*10^6
    Catalog#: YKO-HS1466
  • STK11 Knockout cell line (A549)

    STK11 Knockout cell line (A549)

    Organism: Human
    Gene Name: STK11
    Gene ID: 6794
    Size: 1*10^6
    Catalog#: YKO-HS709

Contact us