TMEM64 Knockout cell line (Hep G2)

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TMEM64 Knockout cell line (Hep G2) Order

TMEM64 Knockout cell line (Hep G2)
Catalog#
YKO-HT7389
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TMEM64 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TMEM64 Knockout cell line (Hep G2)
Species
Human
Gene Name
TMEM64
Gene ID
169200
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript TMEM64-203)
Validation: PCR+Sanger Sequencing
TMEM64 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TMEM64 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TMEM64 gene knockout TMEM64 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TMEM64 Knockout cell line (Hep G2)
TMEM64 Knockout cell line (Hep G2)
Catalog#
YKO-HT7389
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • TMEM64 Knockout cell line (HeLa)

    TMEM64 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: TMEM64
    Gene ID: 169200
    Size: 1*10^6
    Catalog#: YKO-HS10077
    YKO-HS10077(HeLa)
  • TMEM64 Knockout cell line (HCT 116)

    TMEM64 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: TMEM64
    Gene ID: 169200
    Size: 1*10^6
    Catalog#: YKO-HS10076
    YKO-HS10076(HCT 116)
  • TMEM64 Knockout cell line (HEK293)

    TMEM64 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: TMEM64
    Gene ID: 169200
    Size: 1*10^6
    Catalog#: YKO-HS10075
    YKO-HS10075(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • TMEM64 Knockout cell line (HeLa)

    TMEM64 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TMEM64
    Gene ID: 169200
    Size: 1*10^6
    Catalog#: YKO-HS10077
      inquiry   More details
  • TMEM64 Knockout cell line (HCT 116)

    TMEM64 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: TMEM64
    Gene ID: 169200
    Size: 1*10^6
    Catalog#: YKO-HS10076
      inquiry   More details
  • TMEM64 Knockout cell line (HEK293)

    TMEM64 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: TMEM64
    Gene ID: 169200
    Size: 1*10^6
    Catalog#: YKO-HS10075
      inquiry   More details

TMEM64 Knockout cell line (Hep G2) Order

TMEM64 Knockout cell line (Hep G2)
Catalog#
YKO-HT7389
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TMEM64 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TMEM64 Knockout cell line (Hep G2)
Species
Human
Gene Name
TMEM64
Gene ID
169200
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript TMEM64-203)
Validation: PCR+Sanger Sequencing
TMEM64 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TMEM64 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TMEM64 gene knockout TMEM64 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TMEM64 Knockout cell line (Hep G2)
TMEM64 Knockout cell line (Hep G2)
Catalog#
YKO-HT7389
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • TMEM64 Knockout cell line (HeLa)

    TMEM64 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: TMEM64
    Gene ID: 169200
    Size: 1*10^6
    Catalog#: YKO-HS10077
    YKO-HS10077(HeLa)
  • TMEM64 Knockout cell line (HCT 116)

    TMEM64 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: TMEM64
    Gene ID: 169200
    Size: 1*10^6
    Catalog#: YKO-HS10076
    YKO-HS10076(HCT 116)
  • TMEM64 Knockout cell line (HEK293)

    TMEM64 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: TMEM64
    Gene ID: 169200
    Size: 1*10^6
    Catalog#: YKO-HS10075
    YKO-HS10075(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • TMEM64 Knockout cell line (HeLa)

    TMEM64 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TMEM64
    Gene ID: 169200
    Size: 1*10^6
    Catalog#: YKO-HS10077
      inquiry   More details
  • TMEM64 Knockout cell line (HCT 116)

    TMEM64 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: TMEM64
    Gene ID: 169200
    Size: 1*10^6
    Catalog#: YKO-HS10076
      inquiry   More details
  • TMEM64 Knockout cell line (HEK293)

    TMEM64 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: TMEM64
    Gene ID: 169200
    Size: 1*10^6
    Catalog#: YKO-HS10075
      inquiry   More details

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