TNFAIP3 Knockout cell line (A549)

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TNFAIP3 Knockout cell line (A549)

TNFAIP3 Knockout cell line (A549) Order

Catalog#YKO-H783

Price (USD)-

Size 1*10^6

Instruction

Exclusive Red Cotton system(en.rc-crispr.com)was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Cell Info

Catalog YKO-H783
Cell line TNFAIP3 Knockout cell line (A549) Cell Type Human Lung Cancer Cell Line
Morphology Epithelial-like, adherent Passage ratio 1:3~1:5
Culture method 90% F12K+10% FBS
Cryopreservation solution 50%F12K+40%FBS+10%DMSO
Special Note

STR Validation

Gene Information

Official symbol
TNFAIP3
Gene id
7128
Official full symbol
TNF alpha induced protein 3
Also known as
A20, AISBL, OTUD7C, TNFA1P2
This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene.

KO strategy

Knockout region: Exon 2 (based on transcript TNFAIP3-206)
Validation: PCR+Sanger Sequencing

Method

Exclusive Red Cotton system(en.rc-crispr.com)was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

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