TNFRSF1A Knockout cell line (Hep G2)

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location: Home > Products > KO Cell Lines > TNFRSF1A Knockout cell line (Hep G2)
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TNFRSF1A Knockout cell line (Hep G2) Order

TNFRSF1A Knockout cell line (Hep G2)
Catalog#
YKO-HT2221
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TNFRSF1A Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
TNFRSF1A Knockout cell line (Hep G2)
Species
Human
Gene Name
TNFRSF1A
Gene ID
7132
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript TNFRSF1A-201)
Validation: PCR+Sanger Sequencing
TNFRSF1A Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TNFRSF1A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TNFRSF1A gene knockout TNFRSF1A Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TNFRSF1A Knockout cell line (Hep G2)
TNFRSF1A Knockout cell line (Hep G2)
Catalog#
YKO-HT2221
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Organism: Human
    Gene Name: TNFRSF1A
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    Catalog#: YKO-HT0202
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    Organism: Human
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