Tnfrsf1a Knockout cell line (Raw 264.7)

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Tnfrsf1a Knockout cell line (Raw 264.7) Order

Tnfrsf1a Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0115
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Tnfrsf1a Knockout cell line (Raw 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Tnfrsf1a Knockout cell line (Raw 264.7)
Species
Mouse
Gene Name
Tnfrsf1a
Gene ID
21937
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript Tnfrsf1a-201)
Validation: PCR+Sanger Sequencing
Tnfrsf1a Knockout cell line (Raw 264.7) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Tnfrsf1a gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Raw 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Tnfrsf1a gene knockout Tnfrsf1a Knockout cell line (Raw 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Tnfrsf1a Knockout cell line (Raw 264.7)
Tnfrsf1a Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0115
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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