Catalog#:YKO-H540
Price (USD):-
Size: 1*10^6
Exclusive Red Cotton system(en.rc-crispr.com)was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.
Catalog | YKO-H540 | ||
Cell line | UBQLN4 Knockout cell line(U-2 OS) | Cell Type | Mouse Lewis lung carcinoma Cell Line |
Morphology | Epithelial-like, adherent | Passage ratio | 1:3~1:5 |
Culture method | 90% McCOY's 5A+10%FBS | ||
Cryopreservation solution | 50%McCOY's 5A+40%FBS+10%DMSO | ||
Special Note |