UBQLN4 Knockout cell line(Flp-In-TRex U2OS)

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UBQLN4 Knockout cell line(Flp-In-TRex U2OS)

UBQLN4 Knockout cell line(Flp-In-TRex U2OS) Order

Catalog#YKO-H540

Price (USD)-

Size 1*10^6

Instruction

Exclusive Red Cotton system(en.rc-crispr.com)was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Cell Info

Catalog YKO-H540
Cell line UBQLN4 Knockout cell line(U-2 OS) Cell Type Mouse Lewis lung carcinoma Cell Line
Morphology Epithelial-like, adherent Passage ratio 1:3~1:5
Culture method 90% McCOY's 5A+10%FBS
Cryopreservation solution 50%McCOY's 5A+40%FBS+10%DMSO
Special Note

STR Validation

Gene Information

Official symbol
UBQLN4
Gene id
56893
Official full symbol
ubiquilin 4
Also known as
A1U, A1Up, C1orf6, CIP75, UBIN
None

KO strategy

Knockout region: Exon 3 (based on transcript UBQLN4-201)
Validation: PCR+Sanger Sequencing

Method

Exclusive Red Cotton system(en.rc-crispr.com)was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

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