VDR Knockout cell line(Hela)

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VDR Knockout cell line(Hela) Order

VDR Knockout cell line(Hela)
Catalog#
YKO-H357
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
VDR Knockout cell line(Hela)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
VDR Knockout cell line (Hela)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
VDR
Gene ID
7421
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 3 (based on transcript VDR-206)
Validation: PCR+Sanger Sequencing
VDR Knockout cell line(Hela) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:Hela
STR Info (Cell bank)
Cell Line:HELA
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 1213.31213.3
D7S820 812812
D16S539 910910
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 812812
CSF1PO 910910
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the VDR gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hela cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, VDR gene knockout VDR Knockout cell line (Hela) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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