ABL2 Knockout cell line (RD)

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ABL2 Knockout cell line (RD) Order

ABL2 Knockout cell line (RD)
Catalog#
YKO-H1515
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ABL2 Knockout cell line (RD)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
ABL2 Knockout cell line (RD)
Cell Morphology
Epithelial-like, adheren
Passage ratio
1：2-1：3
Species
Human
Gene Name
ABL2
Gene ID
27
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 5 (based on transcript ABL2-203)
Validation: PCR+Sanger Sequencing
ABL2 Knockout cell line (RD) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:RD		 STR Info (Cell bank)
Cell Line:RD
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111111
D13S317 13131313
D7S820 812812
D16S539 10111011
VWA 18181818
TH01 9.39.39.39.3
AMEL XXXX
TPOX 9999
CSF1PO 10111011
D12S391 2020
FGA 2021
D2S1338 2323
D21S11 2829
D18S51 1317
D8S1179 1115
D3S1358 1517
D6S1043 1212
PENTAE 1212
D19S433 1114
PENTAD 1113
D1S1656 1616
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ABL2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RD cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ABL2 gene knockout ABL2 Knockout cell line (RD) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ABL2 Knockout cell line (RD)
ABL2 Knockout cell line (RD)
Catalog#
YKO-H1515
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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