AKAP8 Knockout cell line (Hep G2)

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AKAP8 Knockout cell line (Hep G2) Order

AKAP8 Knockout cell line (Hep G2)
Catalog#
YKO-HT2243
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
AKAP8 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
AKAP8 Knockout cell line (Hep G2)
Species
Human
Gene Name
AKAP8
Gene ID
10270
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript AKAP8-201)
Validation: PCR+Sanger Sequencing
AKAP8 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the AKAP8 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, AKAP8 gene knockout AKAP8 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
AKAP8 Knockout cell line (Hep G2)
AKAP8 Knockout cell line (Hep G2)
Catalog#
YKO-HT2243
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Wild-type Cell Lines

Gene Knockout kit

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  • AKAP8 Knockout cell line (U-2 OS)

    AKAP8 Knockout cell line (U-2 OS)

    Organism: Human
    Gene Name: AKAP8
    Gene ID: 10270
    Size: 1*10^6
    Catalog#: YKO-HT13121
    Request a Quote More details
  • AKAP8 Knockout cell line (Jurkat)

    AKAP8 Knockout cell line (Jurkat)

    Organism: Human
    Gene Name: AKAP8
    Gene ID: 10270
    Size: 1*10^6
    Catalog#: YKO-HT9443
    Request a Quote More details
  • AKAP8 Knockout cell line (HeLa)

    AKAP8 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: AKAP8
    Gene ID: 10270
    Size: 1*10^6
    Catalog#: YKO-XN43656
    Request a Quote More details

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