ANO6 Knockout cell line (Hep G2)

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ANO6 Knockout cell line (Hep G2) Order

ANO6 Knockout cell line (Hep G2)
Catalog#
YKO-HT5117
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ANO6 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
ANO6 Knockout cell line (Hep G2)
Species
Human
Gene Name
ANO6
Gene ID
196527
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 5 (based on transcript ANO6-201)
Validation: PCR+Sanger Sequencing
ANO6 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ANO6 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ANO6 gene knockout ANO6 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ANO6 Knockout cell line (Hep G2)
ANO6 Knockout cell line (Hep G2)
Catalog#
YKO-HT5117
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

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  • ANO6 Knockout cell line (Jurkat)

    ANO6 Knockout cell line (Jurkat)

    Organism: Human
    Gene Name: ANO6
    Gene ID: 196527
    Size: 1*10^6
    Catalog#: YKO-HT9099
    Request a Quote More details
  • ANO6 Knockout cell line (THP-1)

    ANO6 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: ANO6
    Gene ID: 196527
    Size: 1*10^6
    Catalog#: YKO-HT1350
    Request a Quote More details
  • ANO6 Knockout cell line (COS-1)

    ANO6 Knockout cell line (COS-1)

    Organism: Green monkey
    Gene Name: ANO6
    Gene ID: 103238192
    Size: 1*10^6
    Catalog#: YKO-R068
    Request a Quote More details

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