APOE Knockout cell line (HMC3)

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APOE Knockout cell line (HMC3) Order

APOE Knockout cell line (HMC3)
Catalog#
YKO-H1588
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
APOE Knockout cell line (HMC3)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
APOE Knockout cell line (HMC3)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1：2~1：3
Species
Human
Gene Name
APOE
Gene ID
348
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
89%MEM+10%FBS+1% NEAA
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 3 (based on transcript APOE-201)
Validation: PCR+Sanger Sequencing
APOE Knockout cell line (HMC3) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HMC3		 STR Info (Cell bank)
Cell Line:HMC3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11111111
D7S820 911911
D16S539 12131213
VWA 17191719
TH01 6666
AMEL XXXX
TPOX 8989
CSF1PO 10111011
D12S391 1621
FGA 2125
D2S1338 1725
D21S11 3031.2
D18S51 1818
D8S1179 1314
D3S1358 1618
D6S1043 1111
PENTAE 713
D19S433 1515.2
PENTAD 1014
D1S1656 1213
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the APOE gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HMC3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, APOE gene knockout APOE Knockout cell line (HMC3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
APOE Knockout cell line (HMC3)
APOE Knockout cell line (HMC3)
Catalog#
YKO-H1588
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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