ARID1A Knockout cell line (MFE-280)

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ARID1A Knockout cell line (MFE-280) Order

ARID1A Knockout cell line (MFE-280)
Catalog#
YKO-H1425
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ARID1A Knockout cell line (MFE-280)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
ARID1A Knockout cell line (MFE-280)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：3
Species
Gene Name
ARID1A
Gene ID
8289
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%MEM+10%FBS+1%NEAA+1%1%Sodium Pyruvate
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 2 (based on transcript ARID1A-201)
Validation: PCR+Sanger Sequencing
ARID1A Knockout cell line (MFE-280) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MFE-280		 STR Info (Cell bank)
Cell Line:MFE-280
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 10121012
D7S820 10101010
D16S539 12121212
VWA 17171717
TH01 7777
AMEL XXXX
TPOX 910910
CSF1PO 912912
D12S391 2222
FGA 2124
D2S1338 1920
D21S11 3030
D18S51 1318
D8S1179 1113
D3S1358 1818
D6S1043 1416
PENTAE 713
D19S433 1316
PENTAD 99
D1S1656 1516
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ARID1A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MFE-280 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ARID1A gene knockout ARID1A Knockout cell line (MFE-280) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ARID1A Knockout cell line (MFE-280)
ARID1A Knockout cell line (MFE-280)
Catalog#
YKO-H1425
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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