ATP13A2 基因敲除细胞 (HeLa)

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ATP13A2 Knockout cell line (HeLa) Order

ATP13A2 基因敲除细胞 (HeLa)
Catalog#
YKO-H573
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ATP13A2 基因敲除细胞 (HeLa)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
ATP13A2 Knockout cell line (HeLa)
Cell Morphology
上皮样, 贴壁细胞
Passage ratio
1:2-1:4
Species
Human
Gene Name
ATP13A2
Gene ID
23400
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
收录情况
该细胞已被Expasy
ExPASy Cellosaurus数据库
该在线资源收录了ATCC、ECACC、DSMZ等多个权威细胞库的细胞系信息,可提供10万+细胞系详尽数据,包括细胞系描述、来源、特性等,具有广泛的参考价值和科学可信度。它还提供细胞系认证、STR数据及潜在污染风险等信息,帮助研究人员确认其实验中所使用的细胞系的可靠性。
收录: CVCL_D9Y7
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: E5 (based on transcript ATP13A2-201)
Validation: PCR+Sanger Sequencing
ATP13A2 基因敲除细胞 (HeLa) Target region
Loci 送检细胞STR信息
送检细胞名:HeLa
细胞库细胞STR信息
细胞库细胞名:HELA
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 1213.31213.3
D7S820 812812
D16S539 910910
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 812812
CSF1PO 910910
D12S391 202425.0
FGA 2121
D2S1338 1717
D21S11 2728
D18S51 1616
D8S1179 1213
D3S1358 1518
D6S1043 1819
PENTAE 717
D19S433 1314
PENTAD 815
D1S1656 1215
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

采用独家红棉系统(www.rc-crispr.com)设计ATP13A2基因的sgRNA片段敲除方案,将两条sgRNA构建到CRISPR-U™载体上,通过电转法/病毒法将载体转染进HeLa细胞中,使用极限稀释法制备单克隆,并利用EZ-editor™单克隆基因型鉴定试剂盒(货号:YK-MV-1000)对单克隆进行核酸裂解及PCR扩增,再通过Sanger 测序验证基因型,复检合格后对ATP13A2基因敲除HeLa细胞进行扩增和冻存。
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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    Organism: Human
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    ATP13A2 Knockout cell line (HCT 116)

    Organism: Human
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    Size: 1*10^6
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