Acss2 Knockout cell line (H9c2)

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Acss2 Knockout cell line (H9c2) Order

Acss2 Knockout cell line (H9c2)
Catalog#
YKO-R090
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Acss2 Knockout cell line (H9c2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Acss2 Knockout cell line (H9c2)
Species
Rat
Gene Name
Acss2
Gene ID
311569
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 3 (based on transcript Acss2-203)
Validation: PCR+Sanger Sequencing
Acss2 Knockout cell line (H9c2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Acss2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H9c2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Acss2 gene knockout Acss2 Knockout cell line (H9c2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Acss2 Knockout cell line (H9c2)
Acss2 Knockout cell line (H9c2)
Catalog#
YKO-R090
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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