Cd38 Knockout cell line (B16-F10)

EN CN
Scientist.com
Approved Supplier
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
ENCN
location： Home > Products > KO Cell Lines > Cd38 Knockout cell line (B16-F10)
KO Cell Lines
Stable Cell Lines
Wild-type Cell Lines
Stem Cell Lines
All products
  • All products
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • OVA Stable Cell Lines
  • Stable Cell Lines

Cd38 Knockout cell line (B16-F10) Order

Cd38 Knockout cell line (B16-F10)
Catalog#
YKO-M282
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Cd38 Knockout cell line (B16-F10)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Cd38 Knockout cell line (B16-F10)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Mouse
Gene Name
Cd38
Gene ID
12494
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Target region
Knockout region: Exon 1 (based on transcript Cd38-201)
Validation: PCR+Sanger Sequencing
Cd38 Knockout cell line (B16-F10) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:B16-F10		 STR Info (Cell bank)
Cell Line:B16-F10
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 15.016.015.016.0
4-2 20.321.320.321.3
6-7 15.015.0
19-2 13.014.013.014.0
1-2 19.020.019.020.0
7-1 26.226.2
8-1 16.017.016.017.0
1-1 17.018.019.017.018.019.0
3-2 14.015.014.015.0
2-1 16.016.0
15-3 22.323.322.323.3
6-4 18.019.018.019.0
13-1 17.118.117.118.1
11-2 16.017.016.017.0
17-2 15.016.017.015.016.017.0
12-1 17.018.017.018.0
5-5 16.020.016.020.0
X-1 28.028.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Cd38 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the B16-F10 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Cd38 gene knockout Cd38 Knockout cell line (B16-F10) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Cd38 Knockout cell line (B16-F10)
Cd38 Knockout cell line (B16-F10)
Catalog#
YKO-M282
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • CD38 Knockout cell line (Jurkat)

    CD38 Knockout cell line (Jurkat)
    Request a Quote More details

    Organism: Human
    Gene Name: CD38
    Gene ID: 952
    Size: 1*10^6
    Catalog#: YKO-HT9396
    YKO-HT9396(Jurkat)
  • CD38 Knockout cell line (THP-1)

    CD38 Knockout cell line (THP-1)
    Request a Quote More details

    Organism: Human
    Gene Name: CD38
    Gene ID: 952
    Size: 1*10^6
    Catalog#: YKO-HT0429
    YKO-HT0429(THP-1)
  • CD38 Knockout cell line (A549)

    CD38 Knockout cell line (A549)
    Request a Quote More details

    Organism: Human
    Gene Name: CD38
    Gene ID: 952
    Size: 1*10^6
    Catalog#: YKO-HS5074
    YKO-HS5074(A549)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines


WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now

Online
Inquire

Online Inquire

✖

Contact us

  If email is not available, how else can we reach you?
Search Engine
Email
Recommendations from others
Others
✖

Contact us

If email is not available, how else can we reach you?
Search Engine
Email
Recommendations from others
Others
✖
Submission Succeeded
Book A Virtual Meeting With Technical Support